Chumbe-Mendoza A.1,2, Izquierdo-Lara R.1,2, Calderón-Mayo K.1, Fernández-Díaz M.1, Vakharia V.2,3.
1FARVET, Research and Development Laboratories, Ica, Peru. 2Universidad Nacional Mayor de San Marcos, School of Veterinary Medicine, San Borja, Lima, Peru. 3Institute of Marine & Environmental Technology, University of Maryland Baltimore Country, 701 East Pratt St, Baltimore, MD 21202, USA
Newcastle disease is one of the most important infectious diseases of poultry, caused by the Newcastle disease virus (NDV). The hemagglutination inhibition (HI) assay and ELISA are used to measure protection and immune response. However, these techniques do not corroborate the presence of neutralizing antibodies (nAbs). The objective of this work was to generate a genetically modified NDV to express an enhanced green fluorescent protein (eGFP). This NDV-eGFP reporter virus was used to develop a neutralization test, in which nAbs titers were expressed as the reciprocal of the highest dilution that expressed the eGFP.
A total of 57 serum samples were assayed by conventional NT and eGFP-NT. Additionally, HI and a commercial ELISA kit were used to evaluate with the same set of samples. HI (R2=0.816) and ELISA (R2=0.791) showed correlation with conventional NT, eGFP-NT showed higher correlation (R2=0.994). The eGFP-NT is a simple, rapid and reliable method for
the quantitation of NDV specific nAbs.
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