Morales-Ruiz S.¹, Bendezú J.¹, Choque-Guevara R.¹, Montesinos-Millán R.¹, Requena-Anicama D.^1,2, Choque-Moreau L.¹, Montalván-Avalos A.¹, Fernández-Díaz M.^1,3.
¹FARVET, Research and Development Laboratories, Ica, Peru. ² Bioinformatics Laboratory, Universidad Peruana Cayetano Heredia, Lima, Peru, ³FARVET SPF, Ica, Peru

ABSTRACT

The development of tools that allow rapid detection of Avibacterium paragallinarum, which causes infectious coryza (IC) in chickens, is necessary to avoid disease dissemination and economic losses in poultry. An Av. paragallinarum-specific Ma-4 epitope of the TonB-dependent transporter (TBDT) was selected using bioinformatic tools to immunize a BalbC mouse and to produce monoclonal antibodies to be used in a lateral flow test (LFT) developed for Av. paragallinarum detection. The 1G7G8 monoclonal antibody was able to detect TBDT in Av. paragallinarum cultures (serogroups: A, B and C) by Western blot and indirect ELISA assay. We developed a self-pairing prototype LFT, whose  limit of detection using Av. paragallinarum cultures was 1×104 colony-forming units (CFU)/mL.  Nasal mucus samples from 35 chickens suspected of having infectious coryza were evaluated for the LFT detection capacity and compared with bacterial  isolation (B.I) and polymerase chain reaction (PCR). Thus, comparative indicators such as sensitivity (Se), specificity (Sp), positive predictive value (PPV), negative predictive values (NPV) and the kappa index (K) were obtained. The results indicate that the self-pairing prototype LFT is suitable for the TBDT detection in Av. paragallinarum cultures and in field samples.

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